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Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm

Identifieur interne : 003449 ( Main/Exploration ); précédent : 003448; suivant : 003450

Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm

Auteurs : J. Veramendi [Espagne] ; L. Navarro [Espagne]

Source :

RBID : ISTEX:42EA43675B0A95AA70D0444A49B3DC564AD6510A

Abstract

Abstract: Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 μM 2,4-dichlorophenoxyacetic acid, 14.8 μM N6-(2-isopentenyl)adenine and 3 g l-1 activated charcoal to develop nodular callus after 8 months of culture. Callus was cultured in agar-solidified and stationary or shaken liquid media containing half-strength MS inorganic salts, 3 g l-1 activated charcoal and different sucrose concentrations to study the influence of these factors on somatic embryogenesis. The best conditions for embryo development were culturing in liquid medium shaken at 100 rpm for a period of 2 weeks without sucrose, followed by culture on 3% sucrose.

Url:
DOI: 10.1007/BF00048760


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<div type="abstract" xml:lang="en">Abstract: Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 μM 2,4-dichlorophenoxyacetic acid, 14.8 μM N6-(2-isopentenyl)adenine and 3 g l-1 activated charcoal to develop nodular callus after 8 months of culture. Callus was cultured in agar-solidified and stationary or shaken liquid media containing half-strength MS inorganic salts, 3 g l-1 activated charcoal and different sucrose concentrations to study the influence of these factors on somatic embryogenesis. The best conditions for embryo development were culturing in liquid medium shaken at 100 rpm for a period of 2 weeks without sucrose, followed by culture on 3% sucrose.</div>
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